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American Journal of Clinical Nutrition, Vol 14, 197-201, Copyright © 1964 by The American Society for Clinical Nutrition, Inc.

A Method for Assaying Thiamine Status in Man and Animals

HERMAN BAKER PH.D.1, OSCAR FRANK PH.D.1, JAMES J. FENNELLY M.D.1, and CARROLL M. LEEVY M.D.1

1 From the Division of Hepatic Metabolism and Nutrition, Department of Medicine, Seton Hall College of Medicine, Jersey City, New Jersey

The protozoan flagellate, Ochromonas danica, which responds only to intact thiamine, is used for determining thiamine in biologic fluids and tissues. The method more accurately reflects thiamine status in man and animals than do the chemical and enzymatic procedures. The method yields qualitative results in two days and quantitative results in four to five days.




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Copyright © 1964 by The American Society for Nutrition