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American Journal of Clinical Nutrition, Vol 18, 123-133, Copyright © 1966 by The American Society for Clinical Nutrition, Inc.

A Protozoological Method for Detecting Clinical Vitamin B6 Deficiency

HERMAN BAKER PH.D.1, OSCAR FRANK PH.D.1, MAY NING M.D.1, ROSEMARY A. GELLENE M.D.1, S. H. HUTNER PH.D.1, and CARROLL M. LEEVY M.D.1

1 From the Division of Hepatic Metabolism and Nutrition, Department of Medicine, New Jersey College of Medicine and Dentistry, Jersey City, New Jersey 07304, and Haskins Laboratories, New York, New York

A direct assay of circulating or available levels of vitamin B6 in human biologic fluids and tissues is described. The organism used was the ciliate protozoan Tetrahymena pyriformis, an organism which responds only to the mammalian active vitamers of vitamin B6 and hence is a better measure of the status of the vitamin in man as compared with chemical or enzymatic procedures. Application of the assay in subjects with a variety of metabolic disorders, including two patients maintained on total food and vitamin deprivation and five patients with vitamin B6 deficiency anemia, is described.




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H. Baker, O. Frank, S. H. Hutner, H. F. Eichenwald, and P. C. Fry
Subclinical Malnutrition
Science, July 18, 1969; 165(3890): 313 - 313.
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Copyright © 1966 by The American Society for Nutrition