AJCN Tufts Nutrition Symposium, Boston Sept 24-26
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American Journal of Clinical Nutrition, Vol 20, 968-977, Copyright © 1967 by The American Society for Clinical Nutrition, Inc.

A Rapid Analytical System for Determining Serum Lipids

W. A. KREHL M.D., PH.D.1, ALFREDO LOPEZ-S M.D., PH.D.1, and ELEANOR I. GOOD B.S., M.S.1

1 From the Clinical Research Center, University of Iowa, College of Medicine, Iowa City, Iowa

A useful scheme has been developed for the semiautomatic determination of serum lipids, adapting previously described methods into this scheme. This scheme uses only a single isopropanolic extract requiring only 1-2 ml of serum. By this method, accurate and fast measurements are performed of total cholesterol, free cholesterol, cholesteryl esters, triglycerides, free fatty acids, and esterified fatty acids. Using this scheme, a single, well-organized and trained person can determine the above mentioned lipid studies in 20-30 samples in 1 day; two persons could process about 100 samples daily.

Due to the reproducibility and agreement with the classical methods, together with the small amount of serum required and the speed of operation, we believe this scheme of semiautomatic determinations of plasma lipids to be capable of fulfilling the needs of any laboratory involved in large numbers of lipid determinations.






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Copyright © 1967 by The American Society for Nutrition