American Journal of Clinical Nutrition, Vol 22, 79-86, Copyright © 1969 by The American Society for Clinical Nutrition, Inc.

Composition of Human Chyle Chylomicrons following Single Fat Feedings

¹ From the Institute for Metabolic Research, Highland General Hospital, Oakland, California; and the Lymph Research Laboratory, Akron City Hospital, Akron, Ohio

A meal containing a well-defined fat and a very small amount of radioactively labeled free cholesterol was fed in two consecutive studies to a male patient with cannulation of the left thoracic duct. Chyle was collected for 15 or 16 hr following the meal. Recovery of fed fat was 19-27%, and of fed cholesterol radioactivity, only 2.5-3.6%. Absorbed cholesterol traveled predominantly on the chylomicron fraction of total chyle and was present in both the free and esterified forms. Phosphatidyl choline was the predominant phospholipid of chyle chylomicrons (77 mole % of total phospholipids) followed by phosphatidyl ethanolamine (16%) and sphingomyelin (7%); lysophosphatidyl choline was not detected. The fatty acid composition of the fed fat had some influence on the fatty acid composition of chylomicron cholesterol ester but a lesser influence on the composition of chylomicron phosphatidyl choline and phosphatidyl ethanolamine. Chylomicron sphingomyelin was virtually unaffected by the nature of the fat fed, and the characteristic fatty acids of chain length greater than C₁₈ were present in significant proportions.

This article has been cited by other articles:

				A. Nilsson and R.-D. Duan Absorption and lipoprotein transport of sphingomyelin J. Lipid Res., January 1, 2006; 47(1): 154 - 171. [Abstract] [Full Text] [PDF]