AJCN 19th International Congress of Nutrition
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American Journal of Clinical Nutrition, Vol 25, 1314-1317, Copyright © 1972 by The American Society for Clinical Nutrition, Inc.

Roll-tube techniques for anaerobic bacteria

Lillian V. Holdeman 1 and W. E. C. Moore 1

1 From the Anaerobe Laboratory, Division of Basic Sciences, College of Agriculture, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24060

Procedures for preparation of prereduced media and use of the anaerobic tube method for culture of anaerobes are described. Media have an oxidation-reduction potential of –150 mv and are prepared, stored, and inoculated under oxygen-free gas. Specimens may be streaked on the surface of an agar layer on the wall of the tube. For quantitative work, molten agar is inoculated with dilutions of the specimen before the agar is spun and hardened on the tube wall (pour tube). The method is simple and is recommended for isolation of anaerobic bacteria in clinical or research laboratories. Processing of clinical specimens and intestinal contents are discussed.




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