AJCN Tufts Nutrition Symposium, Boston Sept 24-26
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American Journal of Clinical Nutrition, Vol 27, 1214-1220, Copyright © 1974 by The American Society for Clinical Nutrition, Inc.

Simplified method for the long-term study of turnover and body masses of cholesterol by input-output analysis

Paul Samuel M.D.1 and Sidney Lieberman Ph.D.1

1 From The Rockefeller University, New York, New York 10021; The Arteriosclerosis Research Laboratory, The Long Island Jewish-Hillside Medical Center and its Queens Hospital Center Affiliation, New Hyde Park, New York 10040 and Jamaica, New York 11432, and the Departments of Mathematics and Chemistry, Queens College of the City University of New York, Flushing, New York 11367

A simplified, labor-saving procedure is proposed for the long-term follow-up of plasma cholesterol specific activity decay curves, using computerized input-output analysis. During the initial 2 weeks after tracer injection, frequent (4 to 6 or more) blood samples are obtained. For the following 40 weeks blood is taken only once a month. During the last 4 weeks of the experiment, blood samples are obtained weekly or twice weekly (method A). Seventeen patients were studied for 50 to 66 weeks. When the computer used data points obtained only every fourth week (in the midsection of the curves), the mean error incurred in the parameters of the analysis (compared to the full analysis of weekly data points) were: IT (sum of absorbed dietary and biosynthesized cholesterol); 2.6% smaller; Ma (rapidly exchangeable mass of cholesterol): unchanged; M (minimum value for total exchangeable mass of cholesterol): 5.9% smaller: M-Ma (remaining mass of cholesterol): 9.5% smaller. Another simplified method (method B) consists of weekly follow-up of the decay of specific activity for the initial 12 weeks after tracer injection. During the following (circa) 30 weeks no blood samples are obtained. At the end of this period weekly or twice weekly blood specimens are obtained for 4 weeks. When the results of this procedure were compared to full curve analyses the mean differences were: IT: 2.1% smaller; Ma: unchanged; M: 9.3% smaller; M-Ma: 14.9% smaller.






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Copyright © 1974 by The American Society for Nutrition