AJCN Tufts Nutrition Symposium, Boston Sept 24-26
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American Journal of Clinical Nutrition, Vol 27, 837-839, Copyright © 1974 by The American Society for Clinical Nutrition, Inc.

A simple automated method for the determination of urinary thiamin

Kathleen L. Muiruri M.S.1, Dale R. Romsos Ph.D.1, and James R. Kirk Ph.D.1

1 From the Department of Food Science and Human Nutrition, Michigan State University, East Lansing, Michigan 48824

An automated method for measuring urinary thiamin without isolating the thiamin is described. Two aliquots of sample are treated alike prior to measurement of thiochrome fluorescence, except that in the blank, addition of benzene-sulfonyl chloride and sodium hydroxide blocks oxidation of thiamin to thiochrome. Thus, fluorescence due to nonspecific substances can be subtracted. Quenching is negligible.






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Copyright © 1974 by The American Society for Nutrition