American Journal of Clinical Nutrition, Vol 32, 879-883, Copyright © 1979 by The American Society for Clinical Nutrition, Inc

ORIGINAL RESEARCH COMMUNICATIONS

Determination of urinary 4-pyridoxic acid using high performance liquid chromatography

JF Gregory 3d and JR Kirk

A high performance liquid chromatographic method is presented for the determination of the vitamin B6 metabolite, 4-pyridoxic acid, in urine. Urine samples are treated with trichloroacetic acid to precipitate protein. An aliquot of the supernatant is chromatographed using 0.033 M phosphate buffer containing 5% (v/v) methanol (pH 2.2), a fluorometric detector and a commercial reverse phase octadecylsilica column. The high precision of the method and the absence of interfering compounds have been demonstrated. This method provides a rapid, sensitive, and quantitative technique for the measurement of urinary 4-pyridoxic acid for use in metabolic and nutritional studies.

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