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American Journal of Clinical Nutrition, Vol 40, 952-956, Copyright © 1984 by The American Society for Clinical Nutrition, Inc

ORIGINAL RESEARCH COMMUNICATIONS

Diet, nutrition intake, and metabolism in populations at high and low risk for colon cancer. In situ bromination for the separation of the cholesterol-cholestanol pair in human fecal extracts

N Turjman and PP Nair

Neutral sterols from human feces were fractionated by thin-layer chromatography and by gas-liquid chromatography (GLC). A simple thin- layer chromatography procedure for the separation of cholestanol from cholesterol is described. This technique, involving in situ bromination of sterols on thin-layer chromatography plates, converts cholesterol and other unsaturated sterols to the corresponding dibromides. Under these conditions, the cholestanol-cholesterol pair which is normally indistinguishable on thin-layer chromatography and gas-liquid chromatography, is separated. Final quantification of the sterols was carried out by gas-liquid chromatography.




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Copyright © 1984 by The American Society for Nutrition