American Journal of Clinical Nutrition, Vol 42, 198-206, Copyright © 1985 by The American Society for Clinical Nutrition, Inc

ORIGINAL RESEARCH COMMUNICATIONS

Alterations in adipocyte free fatty acid re-esterification associated with obesity and weight reduction in man

RL Leibel, J Hirsch, EM Berry and RK Gruen

Using a newly developed in vitro technique, the rate of re- esterification of lipolyzed free fatty acids (FFA) in small fragments of human subcutaneous adipose tissue was measured. When related to simultaneous glycerol release, this measure permits the calculation of the molar ratios of glycerol and FFA leaving the adipocyte. In weight- stable, never-obese control subjects the molar ratio of FFA:glycerol leaving the adipocytes is 1.4:1. During fasting, this ratio climbs to 2.7:1, close to the theoretical maximum of 3.0:1. Adipocytes from weight-stable obese subjects do not differ significantly from adipocytes of control in regard to this ratio. However, the adipocytes of weight-stable reduced-obese (RO) subjects display a significantly higher FFA:glycerol ratio than the adipocytes of either control or obese subjects. The presence of this fasting-like physiology in adipose tissue from weight-stable RO subjects is of particular interest since these same individuals have other systemic metabolic and subjective findings compatible with caloric deprivation.

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