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American Journal of Clinical Nutrition, Vol 44, 698-703, Copyright © 1986 by The American Society for Clinical Nutrition, Inc

ORIGINAL RESEARCH COMMUNICATIONS

Determination of urinary 4-pyridoxic acid levels as 4-pyridoxic acid lactone using high-performance liquid chromatography

JB Ubbink, WJ Serfontein, PJ Becker and LS De Villiers

A high-performance liquid chromatography method for quantitation of urinary 4-pyridoxic acid excretion is presented. Urine samples were treated with HCl to form 4-pyridoxic acid lactone. Acid-treated urine samples were diluted and analyzed by cation-exchange chromatography involving post-column alkalinization and fluorescence detection. Absence of interfering fluorescent urinary compounds was demonstrated by analyzing urine samples without lactonization and rechromatography of aliquots of 4-pyridoxic acid lactone peaks employing reverse-phase chromatography. The method is suitable for determination of urinary 4- pyridoxic acid excretion in nutritional, metabolic, and pharmacokinetic studies.


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S. P Coburn, R. D Reynolds, J D. Mahuren, W. E Schaltenbrand, Y. Wang, K. L Ericson, M. P Whyte, Y. M Zubovic, P. J Ziegler, D. L Costill, et al.
Elevated plasma 4-pyridoxic acid in renal insufficiency
Am. J. Clinical Nutrition, January 1, 2002; 75(1): 57 - 64.
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