AJCN North Carolina Research Campus
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Said, H. M.
Right arrow Articles by Shingleton, J. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Said, H. M.
Right arrow Articles by Shingleton, J. L.
Agricola
Right arrow Articles by Said, H. M.
Right arrow Articles by Shingleton, J. L.

American Journal of Clinical Nutrition, Vol 49, 690-694, Copyright © 1989 by The American Society for Clinical Nutrition, Inc

ORIGINAL RESEARCH COMMUNICATIONS

Intestinal uptake of retinol: enhancement by bovine milk beta- lactoglobulin

HM Said, DE Ong and JL Shingleton
Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN.

The effect of bovine milk beta-lactoglobulin (BLG) on intestinal uptake of retinol was examined in suckling rats with the everted sac technique. Uptake of 0.06 mumol retinol/L bound to BLG (BLG-retinol) was significantly (p less than 0.01) higher than that of 0.06 mumol free retinol/L both in the jejunum and the ileum. The enhancing effect of BLG on retinol uptake was specific because equimolar concentrations of bovine serum albumin and lactoferrin had no effect on retinol uptake. However, serum retinol-binding protein (RBP), which shares structural and conformational similarities with BLG, also enhanced retinol uptake. BLG, BLG-retinol, and RBP-retinol all inhibited the uptake of retinol from BLG-[3H]retinol in a concentration-dependent manner. Uptake of retinol from BLG-retinol was saturable (apparent Km = 5.6 mumol/L, Vmax = 22.7 nmol.g-1.5 min-1), not affected by metabolic inhibitors, and partially temperature dependent (Q10 = 2.77). BLG also significantly (p less than 0.01) enhanced retinol uptake in the intestine of adult rats. These results demonstrate that BLG specifically enhances intestinal uptake of retinol and suggest the possibility of a receptor for BLG-like proteins at the brush border membrane of the enterocyte.


This article has been cited by other articles:


Home page
 J DAIRY SCIHome page
S. Kushibiki, H. Shingu, T. Komatsu, F. Itoh, T. Hayashi, and K. Hodate
Plasma Lipid Concentrations in Preruminant Calves Fed Whole Milk with Whey Protein Isolate
J Dairy Sci, February 1, 2005; 88(2): 653 - 659.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
R. K. Buddington, K. K. Buddington, D.-F. Deng, G.-I. Hemre, and R. P. Wilson
A High Retinol Dietary Intake Increases its Apical Absorption by the Proximal Small Intestine of Juvenile Sunshine Bass (Morone chrysopsxM. saxatilis)
J. Nutr., September 1, 2002; 132(9): 2713 - 2716.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
E. H. Harrison and M. M. Hussain
Mechanisms Involved in the Intestinal Digestion and Absorption of Dietary Vitamin A
J. Nutr., May 1, 2001; 131(5): 1405 - 1408.
[Abstract] [Full Text]

Home page
 Physiol. Rev.Home page
J. Pacha
Development of Intestinal Transport Function in Mammals
Physiol Rev, October 1, 2000; 80(4): 1633 - 1667.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1989 by The American Society for Nutrition