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American Journal of Clinical Nutrition, Vol 54, 1319S-1322S, Copyright © 1991 by The American Society for Clinical Nutrition, Inc

ORIGINAL RESEARCH COMMUNICATIONS

Influence of dietary intakes of erythorbic acid on plasma vitamin C analyses

HE Sauberlich, SM Wood, T Tamura and LE Freeberg
Department of Nutrition Sciences, University of Alabama, Birmingham 35294.

D-Erythorbic acid is an epimer of L-ascorbic acid, but lacks antiscorbutic activity. It is commonly used as a food additive, particularly in processed meat items. Except for high-performance liquid chromatography (HPLC) methodology, the commonly used analytical procedures to measure vitamin C do not distinguish between the two isomers. A study with seven adult women demonstrated that the concentration of erythorbic acid present in food items commonly consumed was sufficient to produce interference in plasma vitamin C analyses. With the meals used, 7-23% of the apparent vitamin C in plasma obtained 2 h after the ingestion of the meals was actually erythorbic acid when analyzed by HPLC. To avoid falsely high plasma- serum vitamin C values as a result of erythorbic acid ingestion, the analyses should be conducted on overnight fasting blood specimens or with the use of an HPLC-amperometric method.




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Copyright © 1991 by The American Society for Nutrition