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American Journal of Clinical Nutrition, Vol 58, 249S-258S, Copyright © 1993 by The American Society for Clinical Nutrition, Inc
REVIEW ARTICLES |
WL Carroll
Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, St Louis.
Recombinant-DNA technology is now commonly used in virtually every aspect of the biological sciences. The purpose of this brief exposition is to provide an outline of the approaches used to identify genes, to isolate the gene of interest, to amplify the gene if necessary, and to clone genes. A short introduction to the principles of separating very large genes is provided, along with a description of an approach to propagating and cloning these large genes.
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