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American Journal of Clinical Nutrition, Vol 59, 1000-1011, Copyright © 1994 by The American Society for Clinical Nutrition, Inc
ORIGINAL RESEARCH COMMUNICATIONS |
AE el-Khoury, NK Fukagawa, M Sanchez, RH Tsay, RE Gleason, TE Chapman and VR Young
Laboratory of Human Nutrition and Clinical Research Center, Massachusetts Institute of Technology, Cambridge 02139.
The validity of tracer-derived estimates of whole-body leucine balance was investigated. Seven healthy young adult subjects received an adequate protein diet for 6 d; at 1800 on the last day, L-[1- 13C]leucine and [15N-15N]urea were given as primed, continuous intravenous infusions for 24 h. Subjects were in a fasting state for the first 12 h and at 0600 on day 7 they then received hourly 10 equal meals to achieve a fed state. Total leucine intake (diet plus tracer) was 89.4 mg.kg-1.d-1. Mean daily leucine oxidation was equivalent to 89.5 +/- 3.3 mg leucine/kg. The predicted daily oxidation rate, from measurements made during the last hour of the fast and the fifth hour of the fed period, was 91.2 +/- 5.8 mg/kg (P = 0.25 from measured). Measured and predicted whole-body leucine balances were 0.76 +/- 2.99 and -0.98 +/- 5.54 mg/kg, respectively (P = 0.25). Urea production exceeded urea excretion by 20%; daily protein oxidation was the same when estimated from leucine oxidation or nitrogen excretion. Thus, the tracer-balance concept is valid, and reliable predictions of total daily leucine oxidation and whole-body leucine balance can be obtained from short-term measurements of leucine oxidation during fasted and fed states.
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D. J. Millward Metabolic Demands for Amino Acids and the Human Dietary Requirement: Millward and Rivers (1988) Revisited J. Nutr., December 1, 1998; 128(12): 2563S - 2563. [Abstract] [Full Text]
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V. R. Young Human Amino Acid Requirements: Counterpoint to Millward and the Importance of Tentative Revised Estimates J. Nutr., September 1, 1998; 128(9): 1570 - 1573. [Full Text]
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