HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Riso, P. Articles by Porrini, M. Search for Related Content PubMed PubMed Citation Articles by Riso, P. Articles by Porrini, M. Agricola Articles by Riso, P. Articles by Porrini, M.

Does tomato consumption effectively increase the resistance of lymphocyte DNA to oxidative damage?^1,2,3

Background: Lycopene, the main carotenoid in tomato, has been shown to be a potent antioxidant in vitro. However, there is no significant evidence of its antioxidant action in vivo.

Objective: We evaluated the effect of tomato intake on plasma carotenoid concentrations and lymphocyte resistance to oxidative stress.

Design: Ten healthy women (divided into 2 groups of 5 subjects each) ate a diet containing tomato purée (providing 16.5 mg lycopene) and a tomato-free diet for 21 d each in a crossover design. Before and after each diet period, plasma carotenoid concentrations and primary lymphocyte resistance to oxidative stress (evaluated by means of single-cell gel electrophoresis) were analyzed.

Results: After the first 21-d experimental period, total plasma lycopene concentrations increased by 0.5 µmol/L (95% CI: 0.14, 0.87) in the group that consumed the tomato diet and decreased by 0.2 µmol/L (95% CI: -0.11, -0.30) in the group that consumed the tomato-free diet (P < 0.001). Tomato consumption also had an effect on cellular antioxidant capacity: lymphocyte DNA damage after ex vivo treatment with hydrogen peroxide decreased by 33% (95% CI: 0.8%, 61%; P < 0.05) and by 42% (95% CI: 5.1%, 78%; P < 0.05) in the 2 groups of subjects after consumption of the tomato diet.

Conclusion: The consumption of tomato products may reduce the susceptibility of lymphocyte DNA to oxidative damage.

Key Words: Tomato • lycopene • carotenoids • oxidative stress • antioxidants • single-cell gel electrophoresis • SCGE • lymphocytes • women

This article has been cited by other articles:

				S. Devaraj, S. Mathur, A. Basu, H. H. Aung, V. T. Vasu, S. Meyers, and I. Jialal A Dose-Response Study on the Effects of Purified Lycopene Supplementation on Biomarkers of Oxidative Stress J. Am. Coll. Nutr., April 1, 2008; 27(2): 267 - 273. [Abstract] [Full Text] [PDF]

				D. J. Waters, S. Shen, H. Xu, S. S. Kengeri, D. M. Cooley, E. C. Chiang, Y. Chen, D. Schlittler, C. Oteham, G. F. Combs Jr., et al. Noninvasive Prediction of Prostatic DNA Damage by Oxidative Stress Challenge of Peripheral Blood Lymphocytes Cancer Epidemiol. Biomarkers Prev., September 1, 2007; 16(9): 1906 - 1910. [Abstract] [Full Text] [PDF]

				D. Sau, S. De Biasi, L. Vitellaro-Zuccarello, P. Riso, S. Guarnieri, M. Porrini, S. Simeoni, V. Crippa, E. Onesto, I. Palazzolo, et al. Mutation of SOD1 in ALS: a gain of a loss of function Hum. Mol. Genet., July 1, 2007; 16(13): 1604 - 1618. [Abstract] [Full Text] [PDF]

				C. I. Gill, S. Haldar, L. A Boyd, R. Bennett, J. Whiteford, M. Butler, J. R Pearson, I. Bradbury, and I. R Rowland Watercress supplementation in diet reduces lymphocyte DNA damage and alters blood antioxidant status in healthy adults Am. J. Clinical Nutrition, February 1, 2007; 85(2): 504 - 510. [Abstract] [Full Text] [PDF]

				X. Zhao, G. Aldini, E. J Johnson, H. Rasmussen, K. Kraemer, H. Woolf, N. Musaeus, N. I Krinsky, R. M Russell, and K.-J. Yeum Modification of lymphocyte DNA damage by carotenoid supplementation in postmenopausal women Am. J. Clinical Nutrition, January 1, 2006; 83(1): 163 - 169. [Abstract] [Full Text] [PDF]

				K. Briviba, S. E. Kulling, J. Moseneder, B. Watzl, G. Rechkemmer, and A. Bub Effects of supplementing a low-carotenoid diet with a tomato extract for 2 weeks on endogenous levels of DNA single strand breaks and immune functions in healthy non-smokers and smokers Carcinogenesis, December 1, 2004; 25(12): 2373 - 2378. [Abstract] [Full Text] [PDF]

				M. B. Schabath, H. B. Grossman, G. L. Delclos, L. M. Hernandez, R. S. Day, B. R. Davis, S. P. Lerner, M. R. Spitz, and X. Wu Dietary Carotenoids and Genetic Instability Modify Bladder Cancer Risk J. Nutr., December 1, 2004; 134(12): 3362 - 3369. [Abstract] [Full Text] [PDF]

				C. I.R. Gill, S. Haldar, S. Porter, S. Matthews, S. Sullivan, J. Coulter, H. McGlynn, and I. Rowland The Effect of Cruciferous and Leguminous Sprouts on Genotoxicity, In vitro and In vivo Cancer Epidemiol. Biomarkers Prev., July 1, 2004; 13(7): 1199 - 1205. [Abstract] [Full Text] [PDF]

				V. Tyssandier, C. Feillet-Coudray, C. Caris-Veyrat, J.-C. Guilland, C. Coudray, S. Bureau, M. Reich, M.-J. Amiot-Carlin, C. Bouteloup-Demange, Y. Boirie, et al. Effect of Tomato Product Consumption on the Plasma Status of Antioxidant Microconstituents and on the Plasma Total Antioxidant Capacity in Healthy Subjects J. Am. Coll. Nutr., April 1, 2004; 23(2): 148 - 156. [Abstract] [Full Text] [PDF]

				F. Nyberg, S.-M. Hou, G. Pershagen, and B. Lambert Dietary fruit and vegetables protect against somatic mutation in vivo, but low or high intake of carotenoids does not Carcinogenesis, April 1, 2003; 24(4): 689 - 696. [Abstract] [Full Text] [PDF]

				O. Kucuk, F. H. Sarkar, Z. Djuric, W. Sakr, M. N. Pollak, F. Khachik, M. Banerjee, J. S. Bertram, and D. P. Wood Jr Effects of Lycopene Supplementation in Patients with Localized Prostate Cancer Experimental Biology and Medicine, November 1, 2002; 227(10): 881 - 885. [Abstract] [Full Text] [PDF]

				P. Bowen, L. Chen, M. Stacewicz-Sapuntzakis, C. Duncan, R. Sharifi, L. Ghosh, H.-S. Kim, K. Christov-Tzelkov, and R. van Breemen Tomato Sauce Supplementation and Prostate Cancer: Lycopene Accumulation and Modulation of Biomarkers of Carcinogenesis Experimental Biology and Medicine, November 1, 2002; 227(10): 886 - 893. [Abstract] [Full Text] [PDF]

				L. Giovannelli, C. Saieva, G. Masala, G. Testa, S. Salvini, V. Pitozzi, E. Riboli, P. Dolara, and D. Palli Nutritional and lifestyle determinants of DNA oxidative damage: a study in a Mediterranean population Carcinogenesis, September 1, 2002; 23(9): 1483 - 1489. [Abstract] [Full Text] [PDF]

				E.-S. Hwang and P. E. Bowen Can the Consumption of Tomatoes or Lycopene Reduce Cancer Risk? Integr Cancer Ther, June 1, 2002; 1(2): 121 - 132. [Abstract] [PDF]

				P. R. Heaton, R. Ransley, C. J. Charlton, S. J. Mann, J. Stevenson, B. H. E. Smith, J. M. Rawlings, and E. J. Harper Application of Single-Cell Gel Electrophoresis (Comet) Assay for Assessing Levels of DNA Damage in Canine and Feline Leukocytes J. Nutr., June 1, 2002; 132(6): 1598S - 1603. [Abstract] [Full Text] [PDF]

				P. R. Heaton, C. F. Reed, S. J. Mann, R. Ransley, J. Stevenson, C. J. Charlton, B. H. E. Smith, E. J. Harper, and J. M. Rawlings Role of Dietary Antioxidants to Protect against DNA Damage in Adult Dogs J. Nutr., June 1, 2002; 132(6): 1720S - 1724. [Abstract] [Full Text] [PDF]

				S.J. Duthie, L. Pirie, A.McE. Jenkinson, and S. Narayanan Cryopreserved versus freshly isolated lymphocytes in human biomonitoring: endogenous and induced DNA damage, antioxidant status and repair capability Mutagenesis, May 1, 2002; 17(3): 211 - 214. [Abstract] [Full Text] [PDF]

				P. Mecocci, M. C. Polidori, A. Cherubini, T. Ingegni, P. Mattioli, M. Catani, P. Rinaldi, R. Cecchetti, W. Stahl, U. Senin, et al. Lymphocyte Oxidative DNA Damage and Plasma Antioxidants in Alzheimer Disease Arch Neurol, May 1, 2002; 59(5): 794 - 798. [Abstract] [Full Text] [PDF]

				L. Chen, M. Stacewicz-Sapuntzakis, C. Duncan, R. Sharifi, L. Ghosh, R. v. Breemen, D. Ashton, and P. E. Bowen Oxidative DNA Damage in Prostate Cancer Patients Consuming Tomato Sauce-Based Entrees as a Whole-Food Intervention J Natl Cancer Inst, December 19, 2001; 93(24): 1872 - 1879. [Abstract] [Full Text] [PDF]

				O. Kucuk, F. H. Sarkar, W. Sakr, Z. Djuric, M. N. Pollak, F. Khachik, Y.-W. Li, M. Banerjee, D. Grignon, J. S. Bertram, et al. Phase II Randomized Clinical Trial of Lycopene Supplementation before Radical Prostatectomy Cancer Epidemiol. Biomarkers Prev., August 1, 2001; 10(8): 861 - 868. [Abstract] [Full Text] [PDF]

				R. van den Berg, T. van Vliet, W. M. R. Broekmans, N. H. P. Cnubben, W. H. J. Vaes, L. Roza, G. R.M.M. Haenen, A. Bast, and H. van den Berg A Vegetable/Fruit Concentrate with High Antioxidant Capacity Has No Effect on Biomarkers of Antioxidant Status in Male Smokers J. Nutr., June 1, 2001; 131(6): 1714 - 1722. [Abstract] [Full Text]

				W. Stahl, U. Heinrich, S. Wiseman, O. Eichler, H. Sies, and H. Tronnier Dietary Tomato Paste Protects against Ultraviolet Light-Induced Erythema in Humans J. Nutr., May 1, 2001; 131(5): 1449 - 1451. [Abstract] [Full Text]

				A. Bub, B. Watzl, L. Abrahamse, H. Delincée, S. Adam, J. Wever, H. Müller, and G. Rechkemmer Moderate Intervention with Carotenoid-Rich Vegetable Products Reduces Lipid Peroxidation in Men J. Nutr., September 1, 2000; 130(9): 2200 - 2206. [Abstract] [Full Text]

				B. Watzl, A. Bub, M. Blockhaus, B. M. Herbert, P. M. Lührmann, M. Neuhäuser-Berthold, and G. Rechkemmer Prolonged Tomato Juice Consumption Has No Effect on Cell-Mediated Immunity of Well-Nourished Elderly Men and Women J. Nutr., July 1, 2000; 130(7): 1719 - 1723. [Abstract] [Full Text]

				M. Porrini and P. Riso Lymphocyte Lycopene Concentration and DNA Protection from Oxidative Damage Is Increased in Women after a Short Period of Tomato Consumption J. Nutr., January 1, 2000; 130(2): 189 - 192. [Abstract] [Full Text]