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American Journal of Clinical Nutrition, Vol 7, 197-202, Copyright © 1959 by The American Society for Clinical Nutrition, Inc.

A Method for the Determination of Carotene and Vitamin A in Human Blood Serum

OSWALD A. ROELS PH.D.1 and MARIAN TROUT B.SC.1

1 From the Nutrition Laboratory, Institute for Scientific Research in Central Africa (I.R.S.A.C.), Lwiro Bukavu, Belgian Congo

A method for determining total carotene and vitamin A in human serum has been described in detail. The usual procedure of protein precipitation with ethanol, saponification and petroleum ether extraction was followed. Total carotene was measured by a transmission reading at 452 mµ. Vitamin A was determined according to the Carr-Price method with transmission reading at 620 mµ and correction for carotene interference.

Solvents, standards, reagents, and apparatus used have been described because they may greatly influence the results. The calibration graphs and details of the method for calculating the results have been given.






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Copyright © 1959 by The American Society for Nutrition