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Original Research Communications |
1 From the Department of Haematology, St James's Hospital, Dublin, and the Centers for Disease Control and Prevention, Atlanta.
Background: Dried blood spots (DBS) on filter paper have been a successful and economical matrix for neonatal screening.
Objective: Our objective was to develop and evaluate an optimized method for DBS folate analysis and to assess DBS folate stability.
Design: DBS were eluted from paper by sonication in 5 g ascorbic acid/L containing 0.1% (by vol) Triton X-100 and hemoglobin folate values (HF; as pmol/g) were calculated from DBS eluate folate and hemoglobin concentrations.
Results: Over 95% of DBS folate was eluted during a standardized sonication cycle and DBS folate assay reproducibility was acceptable both within (CV: <8%) and between (CV: <9%) runs. HF means (±1 SD) from finger-stick DBS and conventional venous methods were 2513 ± 1144 and 2607 ± 1195 pmol/g, respectively, in blood samples taken concurrently from 80 donors, and they correlated well (r = 0.97, P < 0.001). HF values and erythrocyte folate measures may be interconverted by using the mean cell hemoglobin concentration.
Conclusion: The DBS matrix has potential as an inexpensive and practical option for folate screening studies.
Key Words: Dried blood spot • DBS • red cell folate • erythrocyte folate • hemoglobin concentration • folate concentration • stability • field studies • hemoglobin folate
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