HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Paetau, I. Articles by Clevidence, B. A Search for Related Content PubMed PubMed Citation Articles by Paetau, I. Articles by Clevidence, B. A Agricola Articles by Paetau, I. Articles by Clevidence, B. A

Carotenoids in human buccal mucosa cells after 4 wk of supplementation with tomato juice or lycopene supplements^1,2,3,4

¹ From the US Department of Agriculture, Agricultural Research Service, Beltsville Human Nutrition Research Center, Phytonutrients Laboratory, Beltsville, MD.

Background: Lycopene has been identified as a phytochemical with potentially protective health benefits.

Objective: Our objective was to monitor lycopene changes in buccal mucosa cells (BMCs) in response to 3 vehicles for oral delivery of lycopene.

Design: Fifteen healthy subjects ingested lycopene-rich tomato juice, tomato oleoresin, lycopene beadlets (each containing 70–75 mg lycopene) and a placebo for 4 wk each in a randomized crossover design while consuming self-selected diets. A 6-wk washout period separated the treatment periods. BMCs were collected at baseline and after 4 wk of supplementation.

Results: Lycopene in BMCs increased significantly (2-fold) after 4 wk of ingestion of oleoresin and of beadlets to 4.95 (P < 0.001) and 3.75 µg/g protein (P = 0.053), respectively, but was not significantly affected by tomato juice treatment. The placebo treatment produced a significant decrease in BMC lycopene concentrations (P = 0.018). We observed significant treatment differences between oleoresin and tomato juice, oleoresin and placebo, and beadlets and placebo. BMC concentrations of phytofluene and ß-carotene, which were present in small amounts in the lycopene-containing treatments, increased significantly with ingestion of these products. Strong correlations were found between plasma and BMC concentrations of lutein, ß-cryptoxanthin, -carotene, and ß-carotene. In contrast, correlations between lycopene concentrations in plasma and in BMCs were weak and not significant for any treatment.

Conclusions: The cellular content of lycopene and other tomato-related carotenoids with proposed beneficial health effects can be increased through prolonged supplementation.

Key Words: Lycopene • phytofluene • carotenoids • buccal cells • plasma • tomato juice • oleoresin • beadlets • humans

This article has been cited by other articles:

				A. R. Kristal and J. M. Schenk Directions for Future Epidemiological Research in Lycopene and Prostate Cancer Risk J. Nutr., August 1, 2005; 135(8): 2037S - 2039S. [Full Text] [PDF]

				M. Richelle, K. Bortlik, S. Liardet, C. Hager, P. Lambelet, M. Baur, L. A. Applegate, and E. A. Offord A Food-Based Formulation Provides Lycopene with the Same Bioavailability to Humans as That from Tomato Paste J. Nutr., March 1, 2002; 132(3): 404 - 408. [Abstract] [Full Text] [PDF]