HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) An erratum has been published Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pignatelli, P. Articles by Violi, F. Search for Related Content PubMed PubMed Citation Articles by Pignatelli, P. Articles by Violi, F. Agricola Articles by Pignatelli, P. Articles by Violi, F.

The flavonoids quercetin and catechin synergistically inhibit platelet function by antagonizing the intracellular production of hydrogen peroxide^1,2

¹ From the Department of Experimental Medicine and Pathology, Institute of 1st Clinical Medicine, University La Sapienza, National Institute for Nutrition, Rome.

Background: Epidemiologic studies have shown an inverse relation between moderate consumption of red wine and cardiovascular disease. Studies have shown that red wine and its component flavonoids inhibit in vivo platelet activation, but the underlying mechanism has not yet been identified.

Objective: Because we showed previously that collagen-induced platelet aggregation is associated with a burst of hydrogen peroxide, which in turn contributes to stimulating the phospholipase C pathway, the aim of this study was to investigate whether flavonoids synergize in inhibiting platelet function and interfere with platelet function by virtue of their antioxidant effect.

Design: We tested the effect of 2 flavonoids, quercetin and catechin, on collagen-induced platelet aggregation and hydrogen peroxide and on platelet adhesion to collagen.

Results: Catechin (50–100 µmol/L) and quercetin (10–20 µmol/L) inhibited collagen-induced platelet aggregation and platelet adhesion to collagen. The combination of 25 µmol catechin/L and 5 µmol quercetin/L, neither of which had any effect on platelet function when used alone, significantly inhibited collagen-induced platelet aggregation and platelet adhesion to collagen. Such a combination strongly inhibited collagen-induced hydrogen peroxide production, calcium mobilization, and 1,3,4-inositol triphosphate formation.

Conclusions: These data indicate that flavonoids inhibit platelet function by blunting hydrogen peroxide production and, in turn, phospholipase C activation and suggest that the synergism among flavonoids could contribute to an understanding of the relation between the moderate consumption of red wine and the decreased risk of cardiovascular disease.

Key Words: Platelet aggregation • flavonoids • hydrogen peroxide • platelet adhesion • phospholipase C • quercetin • catechin • red wine • cardiovascular disease

This article has been cited by other articles:

				I. Erlund, R. Koli, G. Alfthan, J. Marniemi, P. Puukka, P. Mustonen, P. Mattila, and A. Jula Favorable effects of berry consumption on platelet function, blood pressure, and HDL cholesterol Am. J. Clinical Nutrition, February 1, 2008; 87(2): 323 - 331. [Abstract] [Full Text] [PDF]

				J. Lin, K. M. Rexrode, F. Hu, C. M. Albert, C. U. Chae, E. B. Rimm, M. J. Stampfer, and J. E. Manson Dietary Intakes of Flavonols and Flavones and Coronary Heart Disease in US Women Am. J. Epidemiol., June 1, 2007; 165(11): 1305 - 1313. [Abstract] [Full Text] [PDF]

				P. Pignatelli, S. Di Santo, B. Buchetti, V. Sanguigni, A. Brunelli, and F. Violi Polyphenols enhance platelet nitric oxide by inhibiting protein kinase C-dependent NADPH oxidase activation: effect on platelet recruitment FASEB J, June 1, 2006; 20(8): 1082 - 1089. [Abstract] [Full Text] [PDF]

				R. Hirano-Ohmori, R. Takahashi, Y. Momiyama, H. Taniguchi, A. Yonemura, S. Tamai, K. Umegaki, H. Nakamura, K. Kondo, and F. Ohsuzu Green Tea Consumption and Serum Malondialdehyde-Modified LDL Concentrations in Healthy Subjects J. Am. Coll. Nutr., October 1, 2005; 24(5): 342 - 346. [Abstract] [Full Text] [PDF]

				R. M. Lamuela-Raventos, A. I. Romero-Perez, C. Andres-Lacueva, and A. Tornero Review: Health Effects of Cocoa Flavonoids Food Science and Technology International, June 1, 2005; 11(3): 159 - 176. [Abstract] [PDF]

				C. L Keen, R. R Holt, P. I Oteiza, C. G Fraga, and H. H Schmitz Cocoa antioxidants and cardiovascular health Am. J. Clinical Nutrition, January 1, 2005; 81(1): 298S - 303S. [Abstract] [Full Text] [PDF]

				H. Sies, T. Schewe, C. Heiss, and M. Kelm Cocoa polyphenols and inflammatory mediators Am. J. Clinical Nutrition, January 1, 2005; 81(1): 304S - 312S. [Abstract] [Full Text] [PDF]

				G. I. Togna, A. R. Togna, M. Franconi, C. Marra, and M. Guiso Olive Oil Isochromans Inhibit Human Platelet Reactivity J. Nutr., August 1, 2003; 133(8): 2532 - 2536. [Abstract] [Full Text] [PDF]

				K. J Murphy, A. K Chronopoulos, I. Singh, M. A Francis, H. Moriarty, M. J Pike, A. H Turner, N. J Mann, and A. J Sinclair Dietary flavanols and procyanidin oligomers from cocoa (Theobroma cacao) inhibit platelet function Am. J. Clinical Nutrition, June 1, 2003; 77(6): 1466 - 1473. [Abstract] [Full Text] [PDF]

				D. Shanmuganayagam, M. R. Beahm, H. E. Osman, C. G. Krueger, J. D. Reed, and J. D. Folts Grape Seed and Grape Skin Extracts Elicit a Greater Antiplatelet Effect When Used in Combination than When Used Individually in Dogs and Humans J. Nutr., December 1, 2002; 132(12): 3592 - 3598. [Abstract] [Full Text] [PDF]

				J. M Geleijnse, L. J Launer, D. A. van der Kuip, A. Hofman, and J. C. Witteman Inverse association of tea and flavonoid intakes with incident myocardial infarction: the Rotterdam Study Am. J. Clinical Nutrition, May 1, 2002; 75(5): 880 - 886. [Abstract] [Full Text] [PDF]

				F. Violi, P. Pignatelli, F.M. Pulcinelli, J. E. Freedman, C. Parker III, L. Li, J. A. Perlman, B. Frei, V. Ivanov, L. R. Deak, et al. Synergism Among Flavonoids in Inhibiting Platelet Aggregation and H2O2 Production Response Circulation, February 26, 2002; 105 (8): e53 - e53. [Full Text] [PDF]

				M. Yoshizumi, K. Tsuchiya, K. Kirima, M. Kyaw, Y. Suzaki, and T. Tamaki Quercetin Inhibits Shc- and Phosphatidylinositol 3-Kinase-Mediated c-Jun N-Terminal Kinase Activation by Angiotensin II in Cultured Rat Aortic Smooth Muscle Cells Mol. Pharmacol., October 1, 2001; 60(4): 656 - 665. [Abstract] [Full Text] [PDF]

				S. J. Duffy, J. A. Vita, M. Holbrook, P. L. Swerdloff, and J. F. Keaney Jr Effect of Acute and Chronic Tea Consumption on Platelet Aggregation in Patients With Coronary Artery Disease Arterioscler. Thromb. Vasc. Biol., June 1, 2001; 21(6): 1084 - 1089. [Abstract] [Full Text] [PDF]

				M. Misikangas, R. Freese, A. M. Turpeinen, and M. Mutanen High Linoleic Acid, Low Vegetable, and High Oleic Acid, High Vegetable Diets Affect Platelet Activation Similarly in Healthy Women and Men J. Nutr., June 1, 2001; 131(6): 1700 - 1705. [Abstract] [Full Text]