HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Marques-Lopes, I. Articles by Martínez, J A. Search for Related Content PubMed PubMed Citation Articles by Marques-Lopes, I. Articles by Martínez, J A. Agricola Articles by Marques-Lopes, I. Articles by Martínez, J A.

Postprandial de novo lipogenesis and metabolic changes induced by a high-carbohydrate, low-fat meal in lean and overweight men^1,2,3

¹ From the Departments of Physiology and Nutrition and of Food Science, University of Navarra, Pamplona, Spain.

Background: Adjustments of carbohydrate intake and oxidation occur in both normal-weight and overweight individuals. Nevertheless, the contribution of carbohydrates to the accumulation of fat through either reduction of fat oxidation or stimulation of fat synthesis in obesity remains poorly investigated.

Objective: The objective of this study was to assess the postprandial metabolic changes and the fractional hepatic de novo lipogenesis (DNL) induced by a high-carbohydrate, low-fat meal in lean and overweight young men.

Design: A high-carbohydrate, low-fat meal was administered to 6 lean and 7 overweight men after a 17.5-h fast. During the fasting and postprandial periods, energy expenditure (EE), macronutrient oxidation, diet-induced thermogenesis, and serum insulin, glucose, triacylglycerol, and fatty acids were measured. To determine DNL, [1-¹³C]sodium acetate was infused and the mass isotopomer distribution analysis method was applied.

Results: After intake of the high-carbohydrate meal, the overweight men had hyperinsulinemia and higher fatty acid and triacylglycerol concentrations than did the lean men. The overweight group showed a greater EE, whereas there was no significant difference in carbohydrate oxidation between the groups. Nevertheless, the overweight men had a marginally higher protein oxidation and a lower lipid oxidation than did the lean men. DNL was significantly higher before and after meal intake in the overweight men and was positively associated with fasting serum glucose and insulin concentrations. Furthermore, postprandial DNL was positively correlated with body fat mass, EE, and triacylglycerol.

Conclusion: After a high-carbohydrate, low-fat meal, overweight men had a lower fat oxidation and a higher fractional hepatic fat synthesis than did lean men.

Key Words: Obesity • hyperinsulinemia • carbohydrates • fuel metabolism • fractional hepatic de novo lipogenesis • overweight men • lean men • diet-induced thermogenesis • energy expenditure • triacylglycerol • fractional hepatic fat synthesis

This article has been cited by other articles:

				M. T. Timlin, B. R. Barrows, and E. J. Parks Increased Dietary Substrate Delivery Alters Hepatic Fatty Acid Recycling in Healthy Men Diabetes, September 1, 2005; 54(9): 2694 - 2701. [Abstract] [Full Text] [PDF]

				M. Garg, S. Bassilian, C. Bell, S. Lee, and W. N. P. Lee Hepatic de Novo Lipogenesis in Stable Low-Birth-Weight Infants During Exclusive Breast Milk Feedings and During Parenteral Nutrition JPEN J Parenter Enteral Nutr, March 1, 2005; 29(2): 81 - 86. [Abstract] [Full Text] [PDF]

				K. Oh, F. B. Hu, E. Cho, K. M. Rexrode, M. J. Stampfer, J. E. Manson, S. Liu, and W. C. Willett Carbohydrate Intake, Glycemic Index, Glycemic Load, and Dietary Fiber in Relation to Risk of Stroke in Women Am. J. Epidemiol., January 15, 2005; 161(2): 161 - 169. [Abstract] [Full Text] [PDF]

				M. T Timlin and E. J Parks Temporal pattern of de novo lipogenesis in the postprandial state in healthy men Am. J. Clinical Nutrition, January 1, 2005; 81(1): 35 - 42. [Abstract] [Full Text] [PDF]

				K. Oh, W. C. Willett, C. S. Fuchs, and E. L. Giovannucci Glycemic Index, Glycemic Load, and Carbohydrate Intake in Relation to Risk of Distal Colorectal Adenoma in Women Cancer Epidemiol. Biomarkers Prev., July 1, 2004; 13(7): 1192 - 1198. [Abstract] [Full Text] [PDF]

				D. Linden, L. William-Olsson, M. Rhedin, A.-K. Asztely, J. C. Clapham, and S. Schreyer Overexpression of mitochondrial GPAT in rat hepatocytes leads to decreased fatty acid oxidation and increased glycerolipid biosynthesis J. Lipid Res., July 1, 2004; 45(7): 1279 - 1288. [Abstract] [Full Text] [PDF]

				J. A. Martinez, M. S. Corbalan, A. Sanchez-Villegas, L. Forga, A. Marti, and M. A. Martinez-Gonzalez Obesity Risk Is Associated with Carbohydrate Intake in Women Carrying the Gln27Glu {beta}2-Adrenoceptor Polymorphism J. Nutr., August 1, 2003; 133(8): 2549 - 2554. [Abstract] [Full Text] [PDF]

				B. Mittendorfer, B. W. Patterson, S. Klein, and L. S. Sidossis VLDL-triglyceride kinetics during hyperglycemia-hyperinsulinemia: effects of sex and obesity Am J Physiol Endocrinol Metab, April 1, 2003; 284(4): E708 - E715. [Abstract] [Full Text] [PDF]

				B. Mittendorfer, B. W. Patterson, and S. Klein Effect of weight loss on VLDL-triglyceride and apoB-100 kinetics in women with abdominal obesity Am J Physiol Endocrinol Metab, March 1, 2003; 284(3): E549 - E556. [Abstract] [Full Text] [PDF]

				J. Dallongeville, A. Harbis, P. Lebel, C. Defoort, D. Lairon, J.-C. Fruchart, and M. Romon The Plasma and Lipoprotein Triglyceride Postprandial Response to a Carbohydrate Tolerance Test Differs in Lean and Massively Obese Normolipidemic Women J. Nutr., August 1, 2002; 132(8): 2161 - 2166. [Abstract] [Full Text] [PDF]