AJCN EB Program 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Toborek, M.
Right arrow Articles by Hennig, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Toborek, M.
Right arrow Articles by Hennig, B.
Agricola
Right arrow Articles by Toborek, M.
Right arrow Articles by Hennig, B.
American Journal of Clinical Nutrition, Vol. 75, No. 1, 119-125, January 2002
© 2002 American Society for Clinical Nutrition

Original Research Communication

Unsaturated fatty acids selectively induce an inflammatory environment in human endothelial cells1,2,3

Michal Toborek, Yong Woo Lee, Rosario Garrido, Simone Kaiser and Bernhard Hennig

1 From the Departments of Surgery and Animal Sciences, University of Kentucky Medical Center, Lexington, and the University of Potsdam, Potsdam, Germany.

Background: Activation of the vascular endothelium by dietary fatty acids may be among the most critical early events in the development of atherosclerosis. However, the specific effects of fatty acids on inflammatory responses in endothelial cells are not fully understood.

Objective: The present study focused on the induction of inflammatory genes in human endothelial cells exposed to individual dietary fatty acids. Because of the significance of nuclear factor {kappa}B (NF-{kappa}B) and activator protein 1 (AP-1) in the regulation of inflammatory gene expression, we also determined the effects of fatty acids on NF-{kappa}B and AP-1 transcriptional activation.

Design: Human umbilical vein endothelial cells were exposed to dietary mono- and polyunsaturated 18-carbon fatty acids. Transcriptional activation of NF-{kappa}B and AP-1 was determined in human umbilical vein endothelial cells transfected with reporter constructs regulated by these transcription factors. Induction of the inflammatory genes was studied by use of reverse transcriptase–polymerase chain reaction.

Results: Of the fatty acids studied, linoleic acid stimulated NF-{kappa}B and AP-1 transcriptional activation the most. In addition, treatment with this fatty acid markedly enhanced messenger RNA levels of tumor necrosis factor {alpha}, monocyte chemoattractant protein 1, vascular cell adhesion molecule 1, and intercellular adhesion molecule 1. Treatment with linolenic acid stimulated only a moderate induction of the genes encoding for these inflammatory mediators, and exposure to oleic acid either had no effect or resulted in decreased inflammatory gene messenger RNA. In addition, exposure to both linoleic and linolenic acids strongly stimulated induction of the phospholipid hydroperoxide glutathione peroxidase gene.

Conclusion: Specific unsaturated dietary fatty acids, particularly linoleic acid, can selectively stimulate the development of a proinflammatory environment within the vascular endothelium.

Key Words: Fatty acids • inflammatory genes • transcription factors • human endothelial cells • atherosclerosis • nuclear factor {kappa}B • activator protein 1




This article has been cited by other articles:


Home page
 J. Am. Coll. Nutr.Home page
H. Shen, E. Oesterling, A. Stromberg, M. Toborek, R. MacDonald, and B. Hennig
Zinc Deficiency Induces Vascular Pro-Inflammatory Parameters Associated with NF-{kappa}B and PPAR Signaling
J. Am. Coll. Nutr., October 1, 2008; 27(5): 577 - 587.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
B. Bermudez, S. Lopez, Y. M. Pacheco, J. Villar, F. J.G. Muriana, J. D. Hoheisel, A. Bauer, and R. Abia
Influence of postprandial triglyceride-rich lipoproteins on lipid-mediated gene expression in smooth muscle cells of the human coronary artery
Cardiovasc Res, July 15, 2008; 79(2): 294 - 303.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
A. P. Simopoulos
The Importance of the Omega-6/Omega-3 Fatty Acid Ratio in Cardiovascular Disease and Other Chronic Diseases
Experimental Biology and Medicine, June 1, 2008; 233(6): 674 - 688.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
E. Soupene, V. Serikov, and F. A. Kuypers
Characterization of an acyl-coenzyme A binding protein predominantly expressed in human primitive progenitor cells
J. Lipid Res., May 1, 2008; 49(5): 1103 - 1112.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
R. Krogh-Madsen, P. Plomgaard, T. Akerstrom, K. Moller, O. Schmitz, and B. K. Pedersen
Effect of short-term intralipid infusion on the immune response during low-dose endotoxemia in humans
Am J Physiol Endocrinol Metab, February 1, 2008; 294(2): E371 - E379.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
G. Zhao, T. D Etherton, K. R Martin, P. J Gillies, S. G West, and P. M Kris-Etherton
Dietary {alpha}-linolenic acid inhibits proinflammatory cytokine production by peripheral blood mononuclear cells in hypercholesterolemic subjects
Am. J. Clinical Nutrition, February 1, 2007; 85(2): 385 - 391.
[Abstract] [Full Text] [PDF]

Home page
 JPEN J Parenter Enteral NutrHome page
A. Buenestado, J. Cortijo, M.-J. Sanz, Y. Naim-Abu-Nabah, M. Martinez-Losa, M. Mata, A. C. Issekutz, E. Marti-Bonmati, and E. J. Morcillo
Olive Oil-Based Lipid Emulsion's Neutral Effects on Neutrophil Functions and Leukocyte-Endothelial Cell Interactions
JPEN J Parenter Enteral Nutr, July 1, 2006; 30(4): 286 - 296.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
F. Maingrette and G. Renier
Linoleic Acid Increases Lectin-Like Oxidized LDL Receptor-1 (LOX-1) Expression in Human Aortic Endothelial Cells
Diabetes, May 1, 2005; 54(5): 1506 - 1513.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
E. M Hjerkinn, I. Seljeflot, I. Ellingsen, P. Berstad, I. Hjermann, L. Sandvik, and H. Arnesen
Influence of long-term intervention with dietary counseling, long-chain n-3 fatty acid supplements, or both on circulating markers of endothelial activation in men with long-standing hyperlipidemia
Am. J. Clinical Nutrition, March 1, 2005; 81(3): 583 - 589.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
H. Staiger, K. Staiger, N. Stefan, H. G. Wahl, F. Machicao, M. Kellerer, and H.-U. Haring
Palmitate-Induced Interleukin-6 Expression in Human Coronary Artery Endothelial Cells
Diabetes, December 1, 2004; 53(12): 3209 - 3216.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. Ghosh, D. Qi, D. An, T. Pulinilkunnil, A. Abrahani, K.-H. Kuo, R. B. Wambolt, M. Allard, S. M. Innis, and B. Rodrigues
Brief episode of STZ-induced hyperglycemia produces cardiac abnormalities in rats fed a diet rich in n-6 PUFA
Am J Physiol Heart Circ Physiol, December 1, 2004; 287(6): H2518 - H2527.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
G. Zhao, T. D. Etherton, K. R. Martin, S. G. West, P. J. Gillies, and P. M. Kris-Etherton
Dietary {alpha}-Linolenic Acid Reduces Inflammatory and Lipid Cardiovascular Risk Factors in Hypercholesterolemic Men and Women
J. Nutr., November 1, 2004; 134(11): 2991 - 2997.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
V. Saraswathi, G. Wu, M. Toborek, and B. Hennig
Linoleic acid-induced endothelial activation: role of calcium and peroxynitrite signaling
J. Lipid Res., May 1, 2004; 45(5): 794 - 804.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Coll. Nutr.Home page
S. Viswanathan, B. D. Hammock, J. W. Newman, P. Meerarani, M. Toborek, and B. Hennig
Involvement of CYP 2C9 in Mediating the Proinflammatory Effects of Linoleic Acid in Vascular Endothelial Cells
J. Am. Coll. Nutr., December 1, 2003; 22(6): 502 - 510.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
R. A. Velliquette and P. Ernsberger
Contrasting Metabolic Effects of Antihypertensive Agents
J. Pharmacol. Exp. Ther., December 1, 2003; 307(3): 1104 - 1111.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
E. Ros
Dietary cis-monounsaturated fatty acids and metabolic control in type 2 diabetes
Am. J. Clinical Nutrition, September 1, 2003; 78(3): 617S - 625.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
J. M. Roberts, J. L. Balk, L. M. Bodnar, J. M. Belizan, E. Bergel, and A. Martinez
Nutrient Involvement in Preeclampsia
J. Nutr., May 1, 2003; 133(5): 1684S - 1692.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Coll. Nutr.Home page
D. Kritchevsky, S. A. Tepper, S. Wright, S. K. Czarnecki, T. A. Wilson, and R. J. Nicolosi
Cholesterol Vehicle in Experimental Atherosclerosis 24: Avocado Oil
J. Am. Coll. Nutr., February 1, 2003; 22(1): 52 - 55.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
Y. W. Lee, B. Hennig, and M. Toborek
Redox-regulated mechanisms of IL-4-induced MCP-1 expression in human vascular endothelial cells
Am J Physiol Heart Circ Physiol, January 1, 2003; 284(1): H185 - H192.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
J. L. Evans, I. D. Goldfine, B. A. Maddux, and G. M. Grodsky
Oxidative Stress and Stress-Activated Signaling Pathways: A Unifying Hypothesis of Type 2 Diabetes
Endocr. Rev., October 1, 2002; 23(5): 599 - 622.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
J. C Rutledge
Links between food and vascular disease
Am. J. Clinical Nutrition, January 1, 2002; 75(1): 4 - 4.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by The American Society for Nutrition