HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) An erratum has been published Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wood, L. G Articles by Garg, M. L Search for Related Content PubMed PubMed Citation Articles by Wood, L. G Articles by Garg, M. L Agricola Articles by Wood, L. G Articles by Garg, M. L

Increased plasma fatty acid concentrations after respiratory exacerbations are associated with elevated oxidative stress in cystic fibrosis patients^1,2,3

¹ From the Discipline of Nutrition and Dietetics, the University of Newcastle, New South Wales, Australia (LGW and MLG); the Department of Paediatrics, John Hunter Children's Hospital, New South Wales, Australia (DAF and DMC); the Department of Respiratory Medicine, New Children's Hospital, Sydney, New South Wales, Australia (DAF); and the Airway Research Centre, the Department of Respiratory Medicine, John Hunter Hospital, New South Wales, Australia (PGG).

Background: Oxidative stress and depleted antioxidant defenses occur in stable cystic fibrosis patients. During acute infection, the balance between oxidants and antioxidants may be further disturbed.

Objective: We examined the oxidative stress during acute infection in cystic fibrosis patients by measuring 8-iso-prostaglandin F₂ (8-iso-PGF₂) and antioxidant defenses in relation to dietary intake, fatty acid status, immune function, and clinical status.

Design: Plasma concentrations of total 8-iso-PGF₂, vitamins E and C, ß-carotene, zinc, selenium, and copper; plasma fatty acid compositions; erythrocyte glutathione concentrations; glutathione peroxidase and superoxide dismutase activity; sputum glutathione and 8-iso-PGF₂ concentrations; lung function; clinical symptoms; and dietary intake were measured in 15 cystic fibrosis patients before and after 10–14 d of intravenous antibiotic treatment for a pulmonary exacerbation.

Results: After treatment, respiratory status improved (percentage of forced expiratory volume in 1 s: 60 ± 6% at baseline compared with 74 ± 7% after treatment, P = 0.01), quality of well-being improved (P = 0.001), and total plasma 8-iso-PGF₂ concentrations increased from 469 nmol/L at baseline (interquartile range: 373–554 nmol/L) to 565 nmol/L after treatment (interquartile range: 429–689 nmol/L; P = 0.008). Total energy, fat, carbohydrate, and protein intakes per kilogram body weight also increased; however, dietary antioxidant intake was unchanged. Plasma fatty acid concentrations increased after treatment, strongly correlating with plasma 8-iso-PGF₂ concentrations (r = 0.768, P = 0.001). There were no significant changes in white cell counts or plasma concentrations of vitamins E and C or ß-carotene. Erythrocyte glutathione peroxidase activity was reduced after treatment, whereas there was no significant change in superoxide dismutase activity.

Conclusions: Oxidative stress increased after treatment for pulmonary exacerbations and was strongly linked to increased concentrations of plasma fatty acids. Although intravenous antibiotic therapy and physiotherapy improved lung function within 10–14 d of treatment, the biochemical effects of oxidation continued further. Thus, antioxidant intervention during treatment for and recovery from acute infection in cystic fibrosis should be considered.

Key Words: Cystic fibrosis • oxidative stress • exacerbation • antioxidants • isoprostanes • fatty acids • vitamin E • vitamin C • ß-carotene • glutathione peroxidase • superoxide dismutase

This article has been cited by other articles:

				I. Durieu, F. Abbas-Chorfa, J. Drai, J. Iwaz, J-P. Steghens, M. Puget, R. Ecochard, and G. Bellon Plasma fatty acids and lipid hydroperoxides increase after antibiotic therapy in cystic fibrosis Eur. Respir. J., May 1, 2007; 29(5): 958 - 964. [Abstract] [Full Text] [PDF]

				E. I Back, C. Frindt, D. Nohr, J. Frank, R. Ziebach, M. Stern, M. Ranke, and H. K Biesalski Antioxidant deficiency in cystic fibrosis: when is the right time to take action? Am. J. Clinical Nutrition, August 1, 2004; 80(2): 374 - 384. [Abstract] [Full Text] [PDF]

				C. Schupp, E. Olano-Martin, C. Gerth, B. M Morrissey, C. E Cross, and J. S Werner Lutein, zeaxanthin, macular pigment, and visual function in adult cystic fibrosis patients Am. J. Clinical Nutrition, June 1, 2004; 79(6): 1045 - 1052. [Abstract] [Full Text] [PDF]

				L.G. Wood, P.G. Gibson, and M.L. Garg Biomarkers of lipid peroxidation, airway inflammation and asthma Eur. Respir. J., January 1, 2003; 21(1): 177 - 186. [Abstract] [Full Text] [PDF]

				L. G Wood, D. A Fitzgerald, A. K Lee, and M. L Garg Improved antioxidant and fatty acid status of patients with cystic fibrosis after antioxidant supplementation is linked to improved lung function Am. J. Clinical Nutrition, January 1, 2003; 77(1): 150 - 159. [Abstract] [Full Text] [PDF]