HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mohanty, P. Articles by Dandona, P. Search for Related Content PubMed PubMed Citation Articles by Mohanty, P. Articles by Dandona, P. Agricola Articles by Mohanty, P. Articles by Dandona, P.

Both lipid and protein intakes stimulate increased generation of reactive oxygen species by polymorphonuclear leukocytes and mononuclear cells^1,2,3

¹ From the Division of Endocrinology, Diabetes and Metabolism, State University of New York at Buffalo and Kaleida Health, Buffalo.

Background: It was recently shown that glucose challenge leads to increased generation of reactive oxygen species (ROS) by polymorphonuclear leukocytes (PMNs) and mononuclear cells (MNCs).

Objective: To further elucidate the relation between nutrition and ROS generation, we investigated the effect of lipid and protein challenges on ROS generation by leukocytes.

Design: After having fasted overnight, one group of healthy subjects consumed a carbohydrate- and protein-free cream preparation (1257 kJ) and another group of healthy subjects consumed an equienergetic pure preparation of casein. Sequential blood samples were obtained after the intake of cream and casein. ROS were measured by chemiluminescence after stimulation by N-formyl-methionyl-leucinyl-phenylalanine. Lipid peroxidation was measured as thiobarbituric acid–reactive substances (TBARS) and -tocopherol was measured by HPLC.

Results: ROS generation by MNCs and PMNs increased significantly 1, 2, and 3 h after cream intake and 1 h after protein intake. Cholesterol concentrations did not change significantly, whereas triacylglycerol concentrations increased significantly 2 h after cream intake. Total TBARS concentrations increased 1 h after cream intake and remained elevated 3 h after intake, but the increase was not significant when corrected for changes in triacylglycerol. After casein intake, total cholesterol, triacylglycerol, and TBARS concentrations did not change significantly. -Tocopherol concentrations did not change significantly after either cream or casein intake.

Conclusions: Both fat and protein intakes stimulate ROS generation. The increase in ROS generation lasted 3 h after cream intake and 1 h after protein intake. Cream intake also caused a significant and prolonged increase in lipid peroxidation. These data are important because increased ROS generation and lipid peroxidation are key events in atherogenesis.

Key Words: Lipids • proteins • reactive oxygen species • leukocytes • lipid peroxidation • aging • thiobarbituric acid • reactive substances • TBARS • polymorphonuclear leukocytes • mononuclear cells • -tocopherol

This article has been cited by other articles:

				D. Versari, E. Daghini, A. Virdis, L. Ghiadoni, and S. Taddei Endothelial Dysfunction as a Target for Prevention of Cardiovascular Disease Diabetes Care, November 1, 2009; 32(suppl_2): S314 - S321. [Full Text] [PDF]

				R. J. Bloomer and K. Fisher-Wellman The role of exercise in minimizing postprandial oxidative stress in cigarette smokers Nicotine Tob Res, January 1, 2009; 11(1): 3 - 11. [Abstract] [Full Text] [PDF]

				E. Grossman Does Increased Oxidative Stress Cause Hypertension? Diabetes Care, February 1, 2008; 31(Supplement_2): S185 - S189. [Abstract] [Full Text] [PDF]

				H. Ghanim, P. Mohanty, R. Pathak, A. Chaudhuri, C. L. Sia, and P. Dandona Orange Juice or Fructose Intake Does Not Induce Oxidative and Inflammatory Response Diabetes Care, June 1, 2007; 30(6): 1406 - 1411. [Abstract] [Full Text] [PDF]

				M. J van Erk, W. A. Blom, B. van Ommen, and H. F. Hendriks High-protein and high-carbohydrate breakfasts differentially change the transcriptome of human blood cells. Am. J. Clinical Nutrition, November 1, 2006; 84(5): 1233 - 1241. [Abstract] [Full Text] [PDF]

				S. E Kasim-Karakas, A. Tsodikov, U. Singh, and I. Jialal Responses of inflammatory markers to a low-fat, high-carbohydrate diet: effects of energy intake. Am. J. Clinical Nutrition, April 1, 2006; 83(4): 774 - 779. [Abstract] [Full Text] [PDF]

				F. Gonzalez, N. S. Rote, J. Minium, and J. P. Kirwan Increased Activation of Nuclear Factor {kappa}B Triggers Inflammation and Insulin Resistance in Polycystic Ovary Syndrome J. Clin. Endocrinol. Metab., April 1, 2006; 91(4): 1508 - 1512. [Abstract] [Full Text] [PDF]

				F Gonzalez, N S Rote, J Minium, and J P Kirwan In vitro evidence that hyperglycemia stimulates tumor necrosis factor-{alpha} release in obese women with polycystic ovary syndrome. J. Endocrinol., March 1, 2006; 188(3): 521 - 529. [Abstract] [Full Text] [PDF]

				A. R. Gosmanov, F. B. Stentz, and A. E. Kitabchi De novo emergence of insulin-stimulated glucose uptake in human aortic endothelial cells incubated with high glucose Am J Physiol Endocrinol Metab, March 1, 2006; 290(3): E516 - E522. [Abstract] [Full Text] [PDF]

				F. Gonzalez, N. S. Rote, J. Minium, and J. P. Kirwan Reactive Oxygen Species-Induced Oxidative Stress in the Development of Insulin Resistance and Hyperandrogenism in Polycystic Ovary Syndrome J. Clin. Endocrinol. Metab., January 1, 2006; 91(1): 336 - 340. [Abstract] [Full Text] [PDF]

				B. Manuel-y-Keenoy, A. Van Campenhout, P. Aerts, J. Vertommen, P. Abrams, L. F. Van Gaal, C. Van Gils, and I. H. De Leeuw Time Course of Oxidative Stress Status in the Postprandial and Postabsorptive States in Type 1 Diabetes Mellitus: Relationship to Glucose and Lipid Changes J. Am. Coll. Nutr., December 1, 2005; 24(6): 474 - 485. [Abstract] [Full Text] [PDF]

				P. Dandona, A. Aljada, A. Chaudhuri, P. Mohanty, and R. Garg Metabolic Syndrome: A Comprehensive Perspective Based on Interactions Between Obesity, Diabetes, and Inflammation Circulation, March 22, 2005; 111(11): 1448 - 1454. [Full Text] [PDF]

				Z. T. Bloomgarden Dyslipidemia and the Metabolic Syndrome Diabetes Care, December 1, 2004; 27(12): 3009 - 3016. [Full Text] [PDF]

				H. Ghanim, A. Aljada, D. Hofmeyer, T. Syed, P. Mohanty, and P. Dandona Circulating Mononuclear Cells in the Obese Are in a Proinflammatory State Circulation, September 21, 2004; 110(12): 1564 - 1571. [Abstract] [Full Text] [PDF]

				G. Engstrom, B. Hedblad, L. Stavenow, S. Jonsson, P. Lind, L. Janzon, and F. Lindgarde Incidence of Obesity-Associated Cardiovascular Disease Is Related to Inflammation-Sensitive Plasma Proteins: A Population-Based Cohort Study Arterioscler Thromb Vasc Biol, August 1, 2004; 24(8): 1498 - 1502. [Abstract] [Full Text] [PDF]

				A. Aljada, H. Ghanim, P. Mohanty, T. Syed, A. Bandyopadhyay, and P. Dandona Glucose intake induces an increase in activator protein 1 and early growth response 1 binding activities, in the expression of tissue factor and matrix metalloproteinase in mononuclear cells, and in plasma tissue factor and matrix metalloproteinase concentrations Am. J. Clinical Nutrition, July 1, 2004; 80(1): 51 - 57. [Abstract] [Full Text] [PDF]

				A. Aljada, P. Mohanty, H. Ghanim, T. Abdo, D. Tripathy, A. Chaudhuri, and P. Dandona Increase in intranuclear nuclear factor {kappa}B and decrease in inhibitor {kappa}B in mononuclear cells after a mixed meal: evidence for a proinflammatory effect Am. J. Clinical Nutrition, April 1, 2004; 79(4): 682 - 690. [Abstract] [Full Text] [PDF]

				G. Engstrom, B. Hedblad, L. Stavenow, P. Lind, L. Janzon, and F. Lindgarde Inflammation-Sensitive Plasma Proteins Are Associated With Future Weight Gain Diabetes, August 1, 2003; 52(8): 2097 - 2101. [Abstract] [Full Text] [PDF]

				D. E. King, A. G. Mainous III, T. A. Buchanan, and W. S. Pearson C-Reactive Protein and Glycemic Control in Adults With Diabetes Diabetes Care, May 1, 2003; 26(5): 1535 - 1539. [Abstract] [Full Text] [PDF]