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Lipid standardization of serum fat-soluble antioxidant concentrations: the YALTA study^1,2,3

¹ From the Department of Laboratory Medicine and Pathology, School of Medicine (MG), and the Division of Epidemiology, School of Public Health (XY, PH, CP, and DRJ), University of Minnesota, Minneapolis.

Background: Blood lipids can influence fat-soluble antioxidant concentrations and confound their interpretation as indicators of antioxidant intake status and disease risk.

Objectives: The objectives were to identify lipoproteins that can confound the interpretation of serum fat-soluble antioxidants, to evaluate the amount of the confounding, and to recommend a method for standardizing blood concentrations of fat-soluble antioxidants.

Design: Several methods of lipid standardization of fat-soluble antioxidants were evaluated in a large cohort of young adults with the use of both cross-sectional and longitudinal data analysis.

Results: Tocopherol and carotenoid concentrations were associated with plasma total cholesterol and its components, LDL, HDL, and VLDL cholesterol (estimated as plasma total triacylglycerols/5), some of which were independent predictors for all of the fat-soluble antioxidants. Among supplement nonusers, the most amphipathic (polar) of the antioxidants (-tocopherol, -tocopherol, and zeaxanthin plus lutein) and lycopene were associated strongly with these lipid fractions (R² = 0.09, 0.40). Consistent with a causal association in which blood antioxidant concentrations change as blood lipid concentrations change, similar relations were found for changes in blood antioxidant and lipid concentrations over a 7-y period. Concentrations of the remaining carotenoids (ß-cryptoxanthin, -carotene, and ß-carotene) had a weaker association with plasma lipoproteins (R² < 0.06). Similar relations were found for supplement users.

Conclusions: The simultaneous adjustment of the concentrations of tocopherols, zeaxanthin plus lutein, and lycopene for VLDL, HDL, and LDL cholesterol is recommended. This method is practical and can provide a basis for the standardization of carotenoid and tocopherol concentrations.

Key Words: Serum antioxidant concentration • blood lipids • lipid standardization • lipoproteins • total cholesterol • fat-soluble vitamins • young adults

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