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Dietary antioxidants and fat are associated with plasma antibody titers to heat shock proteins 60, 65, and 70 in subjects with dyslipidemia^1,2,3

¹ From the Centre for Clinical Science & Measurement (MG-M, DJL, BJS, CL, TW, and GAF) and the Centre for Nutrition and Food Safety (SAN), School of Biomedical and Molecular Sciences, University of Surrey, Guildford, Surrey, United Kingdom; the Department of Clinical Biochemistry, The Royal Surrey County Hospital, Guildford, United Kingdom (BJS, CL, TW, NV, and GAF); and the Faculty of Medicine, Mashad University of Medical Sciences, Mashad, Iran (MG-M)

Background: The heat shock proteins (HSPs) are protein chaperones. Higher titers of antibody to HSPs (anti-HSPs) have been reported in atherosclerosis, which may contribute to immunoactivation in this process.

Objective: We investigated whether dietary antioxidants and fat intake are associated with changes in anti-HSP titers in dyslipidemic subjects.

Design: Patients (n = 238) were recruited from hospital lipid clinics. Control subjects (n = 188) were recruited from university and hospital employees. Food-frequency questionnaires were used to estimate dietary antioxidants and fat.

Results: Dyslipidemic patients had significantly higher titers of anti-HSPs than did control subjects; expressed in medians and interquartile ranges of absorbance units, anti-HSP-60 titers were 0.27 (0.18–0.37) and 0.22 (0.16–0.30), anti-HSP-65 titers were 0.45 (0.28–0.79) and 0.31 (0.22–0.50), and anti-HSP-70 titers were 0.22 (0.17–0.30) and 0.19 (0.13–0.27), respectively. Median and interquartile ranges of serum concentrations of C-reactive protein [1.25 (0.42–3.26) and 0.58 (0.17–1.42)] and mean (±SEM) concentrations of vitamin E (16.36 ± 0.31 and 14.08 ± 0.38) were also significantly higher in patients than in control subjects, respectively. In dyslipidemic patients, the major dietary predictors of the variability in anti-HSP-60 titers were vitamin C (P = 0.005), vitamin E (P = 0.04), and total fat (P = 0.009) intakes; for anti-HSP-65 titers, vitamin C was the major predictor (P = 0.002). These findings remained significant after adjustment for confounding factors.

Conclusions: Anti-HSP-60, -65, and -70 titers are significantly higher in dyslipidemic patients with or without established coronary disease. Our data indicate an association between dietary constituents and the immune response to HSPs in dyslipidemic subjects.

Key Words: Heat shock proteins • HSP-60 • -65 • and -70 • antibody titers • dietary intake • antioxidants • fat