HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Moughan, P. J Articles by Deglaire, A. Search for Related Content PubMed PubMed Citation Articles by Moughan, P. J Articles by Deglaire, A. Agricola Articles by Moughan, P. J Articles by Deglaire, A.

Dietary peptides increase endogenous amino acid losses from the gut in adults^1,2,3

¹ From the Riddet Centre, Massey University, Palmerston North, New Zealand (PJM and AD), the Fonterra Research Centre, Palmerston North, New Zealand (AMR), and the NZ Institute for Crop & Food Research Ltd, Palmerston North, New Zealand (CAB)

Background: Accurate estimates of endogenous ileal total nitrogen and amino acid flows are necessary to ascertain true dietary amino acid digestibility coefficients and for the factorial estimation of dietary amino acid requirements.

Objective: The objective was to ascertain endogenous amino acid losses from the small bowel in human subjects consuming a protein-free diet or a diet with enzyme-hydrolyzed casein (EHC; MW <5000) as the sole source of nitrogen.

Design: The subjects were 8 men and women with terminal ileum ileostomies after ulcerative colitis who consumed the protein-free and EHC-based diets in a crossover design. Each subject received each test diet in single meals followed by 2 consecutive 9-h total collections of digesta. Digesta samples for the EHC treatment were centrifuged and ultrafiltered (10 000 MW cutoff), with the precipitate-plus-retentate fraction (>10 000 MW) providing a measurement of endogenous ileal amino acids.

Results: The mean endogenous flows for most of the amino acids and nitrogen were significantly (P < 0.05) higher when determined with the EHC-based diet than with the protein-free diet. Mean (n = 8) endogenous ileal nitrogen flows were 2061 and 4233 µg/g dry matter intake for the protein-free and EHC-based diets, respectively.

Conclusion: The traditional protein-free method underestimates endogenous ileal amino acid loss in adults.

Key Words: Adults • endogenous • gut • amino acids • ileum • peptides • obligatory losses

This article has been cited by other articles:

				A. Deglaire, P. J. Moughan, C. Bos, K. Petzke, S. M. Rutherfurd, and D. Tome A Casein Hydrolysate Does Not Enhance Gut Endogenous Protein Flows Compared with Intact Casein When Fed to Growing Rats J. Nutr., March 1, 2008; 138(3): 556 - 561. [Abstract] [Full Text] [PDF]

				M. W. Schaart, A. C. J. M. de Bruijn, D. Tibboel, I. B. Renes, and J. B. van Goudoever Dietary Protein Absorption of the Small Intestine in Human Neonates JPEN J Parenter Enteral Nutr, November 1, 2007; 31(6): 482 - 486. [Abstract] [Full Text] [PDF]

				A. Deglaire, P. J. Moughan, S. M. Rutherfurd, C. Bos, and D. Tome Feeding Dietary Peptides to Growing Rats Enhances Gut Endogenous Protein Flows Compared with Feeding Protein-Free or Free Amino Acid-Based Diets J. Nutr., November 1, 2007; 137(11): 2431 - 2436. [Abstract] [Full Text] [PDF]