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ORIGINAL RESEARCH COMMUNICATION |
1 From the Department of Medicine, Division of Nephrology (BA, ZMM, NDV, and HMS) and the Department of Physiology and Biophysics (NDV and HMS), University of California, Irvine, CA, and the Department of Veterans Affairs Medical Center, Long Beach, CA (BA and HMS)
Background:Folic aid plays an essential role in cellular metabolism. Its deficiency can lead to neural tube defects. However, optimization of body folate homeostasis can reduce the incidence of neural tube defects and may decrease the risk of Alzheimer and cardiovascular diseases and cancer. Hence, food fortification and intake of supplemental folate are widespread.
Objective:We examined the effects of long-term folate oversupplementation on the physiologic markers of intestinal and renal folate uptake processes.
Design:Human-derived intestinal Caco-2 and renal HK-2 epithelial cells were maintained (5 generations) in a growth medium oversupplemented (100 µmol folic acid/L) or maintained under sufficient conditions (0.25 and 9 µmol folic acid/L).
Results:Carrier-mediated uptake of 3H-folic acid (2 µmol/L) at buffer pH 5.5 (but not buffer pH 7.4) by Caco-2 and HK-2 cells maintained under the folate-oversupplemented condition was significantly (P < 0.01) and specifically lower than in cells maintained under the folate-sufficient condition. This reduction in folic acid uptake was associated with a significant decrease in the protein and mRNA levels of the human reduced-folate carrier (hRFC) and a decrease in the activity of the hRFC promoter. It was also associated with a decrease in mRNA levels of the proton-coupled folate transporter/heme carrier protein 1 (PCFT/HCP1) and folate receptor (FR).
Conclusions:Long-term oversupplementation with folate leads to a specific and significant down-regulation in intestinal and renal folate uptake, which is associated with a decrease in message levels of hRFC, PCFT/HCP1, and FR. This regulation appears to be mediated via a transcriptional mechanism, at least for the hRFC system.
Key Words: Folate oversupplementation • intestinal folate uptake • renal folate uptake • hRFC • human reduced-folate carrier • PCFT/HCP1 • proton-coupled folate transporter/heme carrier protein 1 • folate receptor
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