AJCN North Carolina Research Campus
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fuchs, D.
Right arrow Articles by Wenzel, U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fuchs, D.
Right arrow Articles by Wenzel, U.
Agricola
Right arrow Articles by Fuchs, D.
Right arrow Articles by Wenzel, U.
American Journal of Clinical Nutrition, Vol. 86, No. 5, 1369-1375, November 2007
© 2007 American Society for Nutrition

ORIGINAL RESEARCH COMMUNICATION

Proteomic biomarkers of peripheral blood mononuclear cells obtained from postmenopausal women undergoing an intervention with soy isoflavones 1,2,3

Dagmar Fuchs, Katerina Vafeiadou, Wendy L Hall, Hannelore Daniel, Christine M Williams, Joyce H Schroot and Uwe Wenzel

1 From the Department of Food and Nutrition, Molecular Nutrition Unit, Technical University of Munich, Freising, Federal Republic of Germany (DF and HD); Hugh Sinclair Unit of Human Nutrition, Department of Food Biosciences, School of Chemistry, Food Biosciences, and Pharmacy, The University of Reading, United Kingdom (KV, WLH, and CMW); Food Technology Centre, Wageningen, the Netherlands (JHS); and the Molecular Nutrition Research, Interdisciplinary Research Center, University of Giessen, Giessen, Federal Republic of Germany (UW)

Background: The incidence of cardiovascular diseases increases after menopause, and soy consumption is suggested to inhibit disease development.

Objective: The objective was to identify biomarkers of response to a dietary supplementation with an isoflavone extract in postmenopausal women by proteome analysis of peripheral blood mononuclear cells.

Design: The study with healthy postmenopausal woman was performed in a placebo-controlled sequential design. Peripheral mononuclear blood cells were collected from 10 volunteers after 8 wk of receiving daily 2 placebo cereal bars and after a subsequent 8 wk of intervention with 2 cereal bars each providing 25 mg of isoflavones. The proteome of the cells was visualized after 2-dimensional gel electrophoresis, and peptide mass fingerprinting served to identify proteins that by the intervention displayed altered protein concentrations.

Results: Twenty-nine proteins were identified that showed significantly altered expression in the mononuclear blood cells under the soy-isoflavone intervention, including a variety of proteins involved in an antiinflammatory response. Heat shock protein 70 or a lymphocyte-specific protein phosphatase and proteins that promote increased fibrinolysis, such as {alpha}-enolase, were found at increased intensities, whereas those that mediate adhesion, migration, and proliferation of vascular smooth muscle cells, such as galectin-1, were found at reduced intensities after soy extract consumption.

Conclusion: Proteome analysis identified in vivo markers that respond to a dietary intervention with isoflavone-enriched soy extract in postmenopausal women. The nature of the proteins identified suggests that soy isoflavones may increase the antiinflammatory response in blood mononuclear cells that might contribute to the atherosclerosis-preventive activities of a soy-rich diet.

Key Words: Soy • isoflavones • cardiovascular disease • postmenopausal women • peripheral blood mononuclear cells • proteome • heat shock protein 70






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2007 by The American Society for Nutrition