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This Article Full Text (Publish Ahead of Print[PDF]) All Versions of this Article: 90/3/485    most recent ajcn.2009.27814v1 Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Qin, Y. Articles by Ling, W. Search for Related Content PubMed PubMed Citation Articles by Qin, Y. Articles by Ling, W. Agricola Articles by Qin, Y. Articles by Ling, W.

Anthocyanin supplementation improves serum LDL- and HDL-cholesterol concentrations associated with the inhibition of cholesteryl ester transfer protein in dyslipidemic subjects^1,2,3,4

¹ From the Departments of Nutrition (YQ, MX, JM, JL, HYM, LC, and WHL) and Statistics (YTH), School of Public Health, Sun Yat-Sen University (Northern Campus), Guangzhou, China.

² YQ and MX contributed equally to this work.

³ Supported by research grants from the National Natural Science Foundation of China (30730079) and the China Medical Board of New York Inc (CMB 98-677). The treated capsules, including the anthocyanin (Medox) and the placebo, were gifts from Polyphenols AS (Sandnes, Norway).

⁴ Address correspondence to WH Ling, Department of Nutrition, School of Public Health, Sun Yat-Sen University (Northern Campus), 74 Zhongshan Road 2, Guangzhou, Guangdong Province 510080, Peoples Republic of China. E-mail: lingwh{at}mail.sysu.edu.cn.

ABSTRACT

Background: Anthocyanins have been shown to exert benefits on the lipid profile in many animal models. Whether these molecules have similar beneficial effects in humans is currently unknown.

Objective: The objective was to investigate the effects of berry-derived anthocyanin supplements on the serum lipid profile in dyslipidemic patients.

Design: A total of 120 dyslipidemic subjects (age 40–65 y) were given 160 mg anthocyanins twice daily or placebo for 12 wk in a double-blind, randomized, placebo-controlled trial.

Results: Anthocyanin consumption increased HDL-cholesterol concentrations (13.7% and 2.8% in the anthocyanin and placebo groups, respectively; P < 0.001) and decreased LDL-cholesterol concentrations (13.6% and –0.6% in the anthocyanin and placebo groups, respectively; P < 0.001). The cellular cholesterol efflux to serum increased more in the anthocyanin group than in the placebo group (20.0% and 0.2%, respectively; P < 0.001). Anthocyanin supplementation decreased the mass and activity of plasma cholesteryl ester transfer protein (CETP) (10.4% and 6.3% in the anthocyanin group and –3.5% and 1.1% in the placebo group, respectively; P < 0.001). In the anthocyanin group, the change in HDL cholesterol was negatively correlated with the change in CETP activity (r_s = –0.330). The change in LDL cholesterol was positively correlated with the change in CETP mass (r_s = 0.354). The change in cellular cholesterol efflux to serum was positively correlated with the change in HDL cholesterol (r_s = 0.485). In vitro, cyanidin 3-O-β-glucosides dose-dependently lowered CETP activity in human HepG2 cells.

Conclusions: Anthocyanin supplementation in humans improves LDL- and HDL-cholesterol concentrations and enhances the cellular cholesterol efflux to serum. These benefits may be due to the inhibition of CETP.

Received for publication March 22, 2009. Accepted for publication June 23, 2009.